Generator

Part:BBa_M39410:Design

Designed by: Zach Banks   Group: MIT BE 20.20-S12   (2012-05-15)


pBad-controlled tetR generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

pBad [BBa_I0500] was selected as an promoter that has low constitutive expression, but can be activated by a molecule in the growth medium (L-arabinose).


Source

Combination of existing parts

References